Bio

experienced scientist with a focus in the analytical development of bioassays assessing the characterization and release of gene therapy and protein biologics, including methods for determining quantity, identity, purity and activity (cell-based potency assays); background in neuropharmacology and small molecule drug discovery; proficient in molecular and cellular techniques, including cell line engineering; efficient in assay development and qualification; excellent verbal and written communication skills; effective leader of a team of scientists; skilled at project management Technical expertise • Molecular biology: molecular cloning, DNA mutagenesis, PCR, western blot, sandwich immunoassays, and Simple Western, immunoprecipitation • Cellular biology: mammalian and primary cell culture (generate striatal, hippocampal and cortical neuronal cultures from postnatal mice and trigeminal, dorsal root ganglion and enteric neuronal cultures from adult mice), nucleic acid transfection, viral transduction, stable-line generation and characterization (by radioligand binding, flow cytometry, immunofluorescence, and immunocytochemistry), live cell confocal microscopy and in vitro functional assays (including ELISA, BRET, FLIPR, CellKey, In-Cell westerns, Cellomics high content screening, enzyme linked complementation and HTRF, MSD, Delfia, AlphaScreen, BLI) in 96- and 384-well HTS-compatible assay formats • In vivo techniques: use of inbred, knockout and transgenic mouse lines, mouse handling and dosing (i.v., p.o., i.p., s.c., i.c.v., i.p.l.), behavioral models related to antinociception, gastrointestinal transit, respiration, pruritus, locomotion, catalepsy, somatic signs of withdrawal, condition place preference/aversion, depression, and serotonin syndrome, tissue and blood collection, colony maintenance and institutional animal protocol compliance • Software: PLA3.0, ELE, LIMS, GraphPad Prism, ChemDraw, ImageJ