My Ph.D. research was focused on exosome secretion and I was able to develop, execute, and troubleshoot a new cell assay for exosome detection. Incorporating in vivo modeling and The Cancer Genome Atlas (TCGA) exploration, I was able to correlate the secretion of exosomes in non-metastatic breast cancers to the patients outcome. Working on other researches, I performed functional assays to characterize the Chimeric Antigen Receptor (CAR) T cells and CAR NK cells. In collaboration with researchers at MD Anderson Cancer Center, I assessed the killing efficiency of post-infusion CAR T cells by monitoring the real-time killing of single cells and characterizing their metabolisms through mitochondria and lysosome structure. I’m skilled in culturing and fluorescent labeling of mammalian cells, immune cell isolation and stimulation, immunoassays, flow cytometry, cytotoxicity assays, performance, and analysis of single-cell and bulk RNA-seq, analysis of 2D and 3D fluorescent images, and performing plasmid cloning, RNA and DNA isolation.
Head of Technology at CellChorus
Doctor of Philosophy (Ph.D.), Chemical and Biomolecular Engineering